Purpose: Over time, plants have been a major source of medicine to treat and cure various ailments and diseases. In many cases, the dosages and standardization of these herbal products have been so ignored that it results in these products causing damages to certain organs of the body unnoticed. The impact of ethanolic leaf extract of Nauclea latifolia on the testes was determined using Wistar rats.
Method: 500, 1000 and 1500 mg of ethanolic leaf extract of Nauclea latifolia /kg body weight was administered for 21 days to 4 groups of animals (n=10). Blood sample was taken for hormonal assay, testicular weight measured, and histological procedure done, and the tissues stained with PAS.
Results: Result from hormonal assay showed reduced level of testosterone and follicle stimulating hormone. After histological staining using periodic acid Schiff method (PAS) and subsequent examination using a compound microscope, there was a reduced level of glycogen granules in all the Nauclea latifolia groups. Findings from this study also showed that the ethanolic leaf extract of Nauclea latifolia at all doses significantly reduced testicular weight (p<0.05).
Conclusion: This study reveals that Nauclea latifolia is anti-androgenic.
World Health Organization. The Promotion and Development of Traditional Medicine. Geneva: Technical Report Series, 622. 2002.
D.E. Okwu, V. Ezenagu, Evaluation of the Phytochemical Composition of Mango (Mangifera indica Linn) Stem, Bark and Leaves. International Journal of Chemical Science, 6(2): 705-716. 2008.
P. Potier, L. Mangatal, M.T. Le Goff, V.F. Querittle, O. Guenard, F. Lavelle. Relationships Between the Structure of Taxol Analogues and their Anti-biotic Activity. Journal of Medical Chemotherapy, 34, 992-998. 1990.
A. Sofowora. Medicinal Plants and Traditional Medicine in Africa, 2nd edition, Ibadan, Nigeria. Spectrum Books Ltd, p. 130.1993.
Morah F.N.I. Morah. Naucleaidal and Epinaucleidal from Anti-viral Preparation from Nauclea latifolia Jamaican Journal of Science and Technology, 5, 22-24. 1994.
J.O. Kokwaro. Medicinal Plants of East Africa. Nairobi. East Africa Literature Bureau.1976; p. 35.
P. I, Akabue, G.C. Mittal Clinical Evaluation of a Traditional Herbal Practice in Nigeria, Preliminary Report. Journal of Ethnopharmacology, 6(3): 355-359. 1982.
G.L. Boye. Studies on Antimalarial Action of Cryptolepsis Sanguinolenta Extract. Procedures on International Symposium. 1990; 41, 243-251.
W. Maduabunyi. Anti-hepatoxic and Trypanocidal Activities of the Ethanolic Extract of Nauclea latifolia Root Bark. Journal of Herbs, Spices and Medicinal Plants, 3(2): 23-53. 1995.
J. Kerharo. Historic and Ethanopharmacognosic Review on the Belief and Traditional Practices in the Treatment of Sleeping Sickness in West Africa. Bulletin of Social and Medical African Noire Lang FR. 19, 400. 1974.
P.I. Akabue, G.C. Mittal. Clinical Evaluation of a Traditional Herbal Practice in Nigeria, Preliminary Report. Journal of Ethnopharmacology, 6(3): 355-359. 1982.
O.I. Asubiojo, V.P. Guinn, A. Okunuga. Multi-element Analysis of Nigeria Chewing Sticks by Instrumental Neuron Activation Analysis. Journal of Radiology and Analytical Chemistry, 74, 149-156. 1982.
A.A. Elujoba. Female Infertility in the Hands of Traditional Birth Attendants in South-West Nigeria. Fitoterapia, 66(3): 239-248. 1995.
S.A. Adesegun, H.A.B. Coker. Plants used in Traditional Medicine in Nigeria. Journal of Pharmacology, 32, 50-62. 2001.
C. Fernandez de la pradilla . Plantes Medicinales Contre les Hepatitis: 51 Especes Traopicales. Ouagadougou: Polygram Educationaire Librairie Jeunesse d’ Afrique, p. 61. 1982a.
M.E. Lamidi, R. Oliver, L. Faurel. L. Debravwer, E. Nze, G. Balandsard. Quinovic Acid Glycosides from Nauclea Diderichic. Planta Medica, 61, 280-281. 1995.
M. Iwu. Handbook of African Medicinal Plants, 2nd edition, Boca Raton, FL: CRC Press, pp. 28-31. 1993.
Y.B. Chiao, D.H. Vanthiel. Mechanisms that Contribute to Alcohol- induced Hypogonadism in the Male. Alcoholism: Clinical and Experimental Research. 7(2): 131-134.1983.
American Physiological Society. Guiding Principles for Research Involving Animals and Human beings. American Journal of Physiological Regulatory Comprehensive Physiology, 283, 281-283. 2002.
M.S. Joshi, R.Y. Ambaye. Effect of Alkaloids from Vincarosea L. on Spermatogenesis in Male Rats. Indian Journal of Experimental Biology, 6, 256-257. 1968.
S.C. D’ Cruz, P.P. Mathur. Effect of Piperine on the Epididymis of Adult Male Rats. Asian Journal of Andrology, 7, 365 –368. 2005.
F. Le Gac, H. Attramadal, T. Johnsen, V. Hansson. Studies on the Mechanism of Follicle Stimulating Hormone Induces Desensitization of Sertoli Cell Adenylyl Cyclase In Vitro. Biology of Reproduction, 32, 916-924. 1985.
V. Piketty, E. Kara, F. Guillou, E. Reiter, P., Crepieux. Follicle Stimulating Hormone (FSH) Activates Extracellular Signal-regulated Kinase Phosphorylation Independently of Beta-arresting and Dynamic-mediated FSH Receptor Internalization. Reproductive Biology and Endocrinology, 4, 33. 2006.
M.J. Lohse, C. Krasel, R. Winstel, F. Mayor Jr. G-protein Coupled Receptor Kinases. Journal of Urology, 49, 1047-1052. 1996.
P. Grasso, L.E. Reichert. Follicle Stimulating Hormone (FSH) Induces G Protein Dissociation from FSH Receptor G Protein complexes in Reconstituted proteoliposomes. Biochemical and Biophysiological Research Communication, 162, 1214-1221. 1989.
L.B. Russel, J.A. Russell, G.R. Mac Gregor, M. Melstricli. Linkage of Manchette Microtubules to the Nuclear Envelope and Observations of the Role of Manchette in Nuclear Shaping during Spermiogenesis in Rodents. Journal of Anatomy, 192, 97-120. 1991.
A.O. Sofola. Textbook of Human Physiology: 10th edition, New York: Raven Press, 3, 87-873. 2001.
H. Mohri, D. A. Suter, P. D. Brown-Woodman, I.G. White, D.D. Ridley. Identification of the Biochemical Lesion Produced by Alpha-chlorhydrin in Spermatozoa. Nature, 255(5503): 75-77. 1975.
N. C. Bennett, R. A. Gardiner, J. D. Hooper, D.W. Johnson, G. C. Gobe. Molecular Cell Biology of Androgen Receptor Signaling. International Journal of Biochemistry and Cell Biology, 42 (6): 813-825. 2010.
Mowszanic Anti-androgens: Mechanisms and Paradoxical Effects. Annual Endocrinology, 50 (3):189-199. 1989.
C. C. Zouboulis, T. Rabe. Hormonal Anti-androgens in Gene Treatment. Journal of the German Society of Dermatology, 8 (suppl): 560-574. 2010.
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