Effect of Vitamin D on Elastin and Collagen Expression: In Vitro Study of Pelvic Organ Prolapse Prevention

Effect of Vitamin D on Elastin and Collagen Expression: In Vitro Study of Pelvic Organ Prolapse Prevention



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Introduction: Pelvic organ prolapse is defined as abnormal protrusion of the pelvic organ beyond its normal anatomical site. It occurs due to the structural weakness of the connective tissue that plays a role in supporting the uterus on the pelvic floor, specifically elastin and collagen. Our study evaluated the effect of vitamin D [1,25(OH)2D3] in preventing pelvic organ prolapse by aggregating elastin and collagen expression.

Material and Methods: A true experimental research was carried out by assessing the cell cultures of sacro-uterine ligament from female patients who underwent hysterectomy. The cell cultures were divided into groups that were exposed to vitamin D at different concentrations of 100 µM, 200 µM, 400 µM, 800 µM, and control without any exposure. The expression of elastin and collagen was subsequently analyzed using immunofluorescence and ELISA method.

Results: This study showed that exposure to vitamin D significantly affected elastin expression (p-value <0.05). The concentration found to be the most effective to induce elastin expression is at 400 µM. Vitamin D also significantly affected the collagen expression (p-value <0.05), with the concentration found to be the most effective to induce collagen expression is at 800 µM.

Conclusion: This study suggested that vitamin D had a significant positive effect of increasing extracellular matrix expression and potentially become a preventive agent for pelvic organ prolapse. Vitamin D is widely available in tropical countries like Indonesia, so this preparation is considered very easy for Indonesian women to apply.

Keywords: Vitamin D Elastin Collagen Extracellular Matrix
  1. Maher, C., Feiner, B., Baessler, K., et al., “Surgical management of pelvic organ prolapse in women,” The Cochrane Database of Systematic Reviews, no. 4, pp. CD004014, April 2013.
    DOI
     Google Scholar 
  2. Kerkhof, M.H., Hendriks, L., Brölmann, H.A., “Changes in connective tissue in patients with pelvic organ prolapse-a review of the current literature,” International Urogynecology Journal and Pelvic Floor Dysfunction, vol. 20, no. 4, pp. 461–474, April 2008.
    DOI
     Google Scholar 
  3. Wibisono, J., Hermawan, G., “Prolaps Organ Panggul,” Medicinus, vol. 7, no. 1, pp. 27–32, January 2018.
    DOI
     Google Scholar 
  4. Megadhana, I.W., Suwiyoga, K., “Stage III-IV uterine prolapse risk factors: sacrouterine ligaments high estrogen receptor alpha and collagen III expression and low elastin expression,” Bali Medical Journal, vol. 5, no. 1, pp. 91–97, August 2016.
    DOI
     Google Scholar 
  5. Jelovsek, J.E., Maher, C., Barber, M.D., “Pelvic organ prolapse,” Lancet, vol. 369, no. 9566, pp. 1027–1038, March 2007.
    DOI
     Google Scholar 
  6. Jo, H.C., Baek, J.C., Park, J.E., et al., “Clinicopathologic characteristics and treatment patterns of pelvic organ prolapse in South Korea,” The Pan African Medical Journal, vol. 34, no. 14, September 2019.
    DOI
     Google Scholar 
  7. Anne, M.W., “An overview of pelvic organ prolapsed,” in Office Urogynecology, Anne, M.W., Linda, B., Joseph, S., et al.: Mc Graw-Hill, 2004, pp. 189–196.
     Google Scholar 
  8. Maldonado, P.A., Wai, C.Y., “Pelvic Organ Prolapse: New Concepts in Pelvic Floor Anatomy,” Obstetrics and Gynecology Clinics of North America, vol. 43, no. 1, pp. 15–26, March 2016.
    DOI
     Google Scholar 
  9. Salvatore, S., Siesto, G., Serati, M., “Risk factors for recurrence of genital prolapse,” Current Opinion in Obstetrics & Gynecology, vol. 22, no. 5, pp. 420–424, October 2010.
    DOI
     Google Scholar 
  10. Gabriel, B., Denschlag, D., Göbel, H., et al., “Uterosacral ligament in postmenopausal women with or without pelvic organ prolapse,” International Urogynecology Journal and Pelvic Floor Dysfunction, vol. 16, no. 6, pp. 475–479, December 2005.
    DOI
     Google Scholar 
  11. Liu, X., Zhao, Y., Pawlyk, B., et al., “Failure of elastic fiber homeostasis leads to pelvic floor disorders,” The American Journal of Pathology, vol. 168, no. 2, pp. 519–528, February 2006.
    DOI
     Google Scholar 
  12. Machin, S.E., Mukhopadhyay, S., “Pelvic organ prolapse: review of the aetiology, presentation, diagnosis and management,” Menopause International, vol. 17, no. 4, pp. 132–136, December 2011.
    DOI
     Google Scholar 
  13. Chow, D., Rodríguez, L.V., “Epidemiology and prevalence of pelvic organ prolapse,” Current Opinion in Urology, vol. 23, no. 4, pp. 293–298, July 2013.
    DOI
     Google Scholar 
  14. Bao, R., Liu, X., Zhou, J., “Primary culture and identification of human fibroblasts,” Journal of Xinxiang Medical College, vol. 28, no. 5, pp. 577–579, 2011.
     Google Scholar 
  15. Budatha, M., Roshanravan, S., Zheng, Q., et al., “Extracellular matrix proteases contribute to progression of pelvic organ prolapse in mice and humans,” The Journal of Clinical Investigation, vol. 121, no. 5, pp. 2048–2059, May 2011.
    DOI
     Google Scholar 
  16. Drewes, P.G., Yanagisawa, H., Starcher, B., et al., “Pelvic organ prolapse in fibulin-5 knockout mice: pregnancy-induced changes in elastic fiber homeostasis in mouse vagina,” The American Journal of Pathology, vol. 170, no. 2, pp. 578–589, February 2007.
    DOI
     Google Scholar 
  17. Wen, Y., Polan, M.L., Chen, B., “Do extracellular matrix protein expressions change with cyclic reproductive hormones in pelvic connective tissue from women with stress urinary incontinence?,” Human Reproduction, vol. 21, no. 5, pp. 1266–1273, May 2006.
    DOI
     Google Scholar 
  18. Crescioli, C., Morelli, A., Adorini, L., et al., “Human bladder as a novel target for vitamin D receptor ligands,” The Journal of Clinical Endocrinology and Metabolism, vol. 90, no. 2, pp. 962–972, February 2005.
    DOI
     Google Scholar 
  19. Ryan, Z.C., Craig, T.A., McGee-Lawrence, M., et al., “Alterations in vitamin D metabolite, parathyroid hormone and fibroblast growth factor-23 concentrations in sclerostin-deficient mice permit the maintenance of a high bone mass,” The Journal of Steroid Biochemistry and Molecular Biology, vol. 148, pp. 225–231, April 2015.
    DOI
     Google Scholar 
  20. Ghanbari, Z., Karamali, M., Mirhosseini, N., et al., “Vitamin D status in women with pelvic floor disorders: a meta-analysis of observational studies,” Journal of Mid-life Health, vol. 10, no. 2, pp. 57–62, June 2019.
    DOI
     Google Scholar 
  21. Allott, B.S., Dittmer, K.E., Kenyon, A.G., et al., “Preliminary investigation of the effect of treating sheep during pregnancy with a vitamin A, D, E formulation on the incidence of vaginal prolapse,” New Zealand Veterinary Journal, vol. 68, no. 3, pp. 193–197, May 2020.
    DOI
     Google Scholar 
  22. Kaur, H., Bala, R., Nagpal, M., “Role of Vitamin D in urogenital health of geriatric participants,” Journal of Mid-life Health, vol. 8, no. 1, pp. 28–35, March 2017.
    DOI
     Google Scholar 
  23. Moon, Y.J., Choi, J.R., Jeon, M.J., et al., “Alteration of elastin metabolism in women with pelvic organ prolapse,” The Journal of Urology, vol. 185, no. 5, pp. 1786–1792, May 2011.
    DOI
     Google Scholar 
  24. Stevenson, S., Thornton, J., “Effect of estrogens on skin aging and the potential role of SERMs,” Clinical Interventions in Aging, vol. 2, no. 3, pp. 283–297, 2007.
    DOI
     Google Scholar 
  25. Klein, T., Bischoff, R., “Physiology and pathophysiology of matrix metalloproteases,” Amino Acids, vol. 41, no. 2, pp. 271–290, July 2011.
    DOI
     Google Scholar 
  26. Skowrońska, P., Pastuszek, E., Kuczyński, W., et al., “The role of vitamin D in reproductive dysfunction in women - a systematic review,” Annals of Agricultural and Environmental Medicine, vol. 23, no. 4, pp. 671–676, December 2016.
    DOI
     Google Scholar 
  27. Grinnell F., “Fibroblast biology in three-dimensional collagen matrices,” Trends in Cell Biology, vol. 13, no. 5, pp. 264–269, May 2003.
    DOI
     Google Scholar 
  28. Barrea, L., Savanelli, M.C., Di Somma, C., et al., “Vitamin D and its role in psoriasis: An overview of the dermatologist and nutritionist,” Reviews in Endocrine & Metabolic Disorders, vol. 18, no. 2, pp. 195–205, June 2017.
    DOI
     Google Scholar 
  29. Christakos, S., Hewison, M., Gardner, D.G., et al., “Vitamin D: beyond bone,” Annals of the New York Academy of Sciences, vol. 1287, no. 1, pp. 45–58, May 2013.
    DOI
     Google Scholar 

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How to Cite

Rahajeng, ., Nurseta, T., Rahardjo, B., Irwanto, Y., & Suseno, D. A. (2021). Effect of Vitamin D on Elastin and Collagen Expression: In Vitro Study of Pelvic Organ Prolapse Prevention. European Journal of Medical and Health Sciences, 3(1), 37–41. https://doi.org/10.24018/ejmed.2021.3.1.657