Antibiotic Resistance of Staphylococcus spp. Isolated from Sewage in Manaus, Amazonas

##plugins.themes.bootstrap3.article.main##

Staphylococcus spp. have become important human pathogens in recent decades due to the selection of resistant bacteria and the spread of their resistance genes in the environment. This study aimed to evaluate the resistance of Staphylococcus spp. obtained from sewage in the city of Manaus, Amazonas state, Brazil. The isolates were tested for susceptibility to antimicrobials using the Kirby-Bauer method for ampicillin, azithromycin, ciprofloxacin, clindamycin, chloramphenicol, erythromycin, gentamicin, oxacillin, cefoxitin, linezolid, penicillin, rifampicin, sulfazotrim, tetracycline and vancomycin. Among the strains isolated from sewage, the greatest resistance was observed for penicillin and oxacillin, with 100% of isolates resistant to these antibiotics. Some antibiotics had resistant and sensitive strains (ampicillin, clindamycin, erythromycin, cefoxitin, azithromycin). Staphylococcus spp. were identified as sensitive to ciprofloxacin chloramphenicol gentamicin linezolid sulfazotrim tetracycline, vancomycin, with no strain resistant to these antibiotics.

  1. Y. Guo, G. Song, M. Sun, J. Wang, and Y. Wang, “Prevalence and Therapies of Antibiotic-Resistance in Staphylococcus aureus”, Frontiers in Cellular and Infection Microbiology, vol. 10:107, 2020.
    DOI
     Google Scholar 
  2. W. A. McGuinness, N. Malachowa, and F. R. DeLeo, “Vancomycin Resistance in Staphylococcus aureus”, Yale Journal of Biology and Medicine, vol. 90 pp. 269-281, 2017.
     Google Scholar 
  3. K. M. Craft, J. M. Nguyen, L. J. Berg, and S. D. Townsend, “Methicillin-resistant Staphylococcus aureus (MRSA): antibiotic-resistance and the biofilm phenotype” Medicinal Chemistry Communications ,vol 10, pp 1231–1241, 2019.
    DOI
     Google Scholar 
  4. A. M. C. Hamzah, C. C. Yeo, S. M. Puah, K. H. Chua, and C.H. Chew, “Staphylococcus aureus Infections in Malaysia: A Review of Antimicrobial Resistance and Characteristics of the Clinical Isolates, 1990–2017” Antibiotics, vol. 8, 128, 2019.
    DOI
     Google Scholar 
  5. M. B. Said, M. S. Abbassi, P. Gómez, L. Ruiz-Ripa, S. Sghaier, C. Ibrahim, C. Torres, and A. Hassen, “Staphylococcus aureus isolated from wastewater treatment plants in Tunisia: occurrence of human and animal associated lineages”. Journal Water Health, vol.15 (4), pp. 638–643, 2017.
    DOI
     Google Scholar 
  6. A. Zehra, R. Singh, S. Kaur, and J. P. S. Gill, “Molecular characterization of antibiotic-resistant Staphylococcus aureus from livestock (bovine and swine)”, Veterinary World, vol. 10(6), pp. 598-604, 2017.
    DOI
     Google Scholar 
  7. L. M. Sejas, S. Silbert, A. O. Reis, and H. S. Sader, “Evaluation of the quality of the antimicrobial agents disks used in disk-diffusion tests comercially available in Brazil”, Jornal Brasileiro de Patologia e Medicina Laboratorial. vol.39. pp. 27-35, 2003.
    DOI
     Google Scholar 
  8. National Committee for Clinical Laboratory Standards-NCCLS. “Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement”. NCCLS document M100-S24. Wayne, PA, 2014.
     Google Scholar 
  9. F. C. Cabello, H. P. Godfrey, A. Tomova, L. Ivanova, H. Dolz, A. Millanao, A. H. Buschmann, “Antimicrobial use in aquaculture re-examined: Its relevance to antimicrobial resistance and to animal and human health”, Environmental Microbiology, vol. 15, pp. 1917-1942, 2013.
    DOI
     Google Scholar 
  10. W. L. C. Bernardo, M. F. G. boriollo, R. B. Gonçalves, J. F. Höfling, “Staphylococcus aureus ampicillin-resistant from the odontological clinic environment”, Revista do Instituto de Medicina Tropical de São Paulo, vol. 47, pp.19-24, 2005.
    DOI
     Google Scholar 
  11. J. G. César, A. M. Peres, C. P. Neves, E. T. F. Abreu, J. F. Mello, Â. N. Moreira, and K. L. Rodrigues “Microbiological assessment of lettuce salads and antimicrobial resistance of Staphylococcus spp.”, Nutrición Hospitalaria, vol. 32, pp. 2280-2285, 2015.
     Google Scholar 
  12. J. Karzis, I. M. Petzer, E. F. Donkin, V. Naidoo, E. M. C. Etter, “Climatic and Regional antibiotic resistance patterns of Staphylococcus aureus in South African dairy herds”, Onderstepoort Journal of Veterinary Research, vol. 86(1), a1674, 2019.
    DOI
     Google Scholar 
  13. A. P. Basso, P. D. Martins, G. Nachtigall, S. Sand, T. M. Moura, A. P. G. Frazzon, “Antibiotic resistance and enterotoxin genes in Staphylococcus sp. isolates from polluted water in Southern Brazil”, Anais da Academia Brasileira de Ciências, vol. 86(4), pp. 1813-1820, 2014.
    DOI
     Google Scholar 
  14. Y. Cong, S. Yang, and X. Rao, “Vancomycin resistant Staphylococcus aureus infections: A review of case updating and clinical features”, Journal of Advanced Research, vol. 21, pp. 169-176, 2020.
    DOI
     Google Scholar 
  15. C. C. Rossi, M. F. Pereira, and M. G. Marval, “Underrated Staphylococcus species and their role in antimicrobial resistance spreading”, Genetics and Molecular Biology, vol. 43, e20190065, 2020.
    DOI
     Google Scholar 
  16. F. Hosseinkhani, M. T. Buirs, F. Jabalameli, M. Emaneini and W. B. van Leeuwen, “High diversity in SCCmec elements among multidrug-resistant Staphylococcus haemolyticus strains originating from paediatric patients; characterization of a new composite island”, Journal of Medical Microbiology, vol. 67, pp. 915-921, 2018.
    DOI
     Google Scholar 
  17. World Health Organization - WHO (2017) “Global action plan on antimicrobial resistance”. WHO Document Production Services, Geneva.
     Google Scholar 

Downloads

Download data is not yet available.

How to Cite

Nogueira, S. P., Andrade, S. L., Magalhães Jr, P. F. C., & Procopio, R. E. L. (2021). Antibiotic Resistance of Staphylococcus spp. Isolated from Sewage in Manaus, Amazonas. European Journal of Medical and Health Sciences, 3(1), 134–137. https://doi.org/10.24018/ejmed.2021.3.1.597

Search Panel

 Sabrine P. Nogueira
 Google Scholar
 PUBMED
 EJMED Journal


 Suanni L. Andrade
 Google Scholar
 PUBMED
 EJMED Journal


 Paulo F. C. Magalhães Jr
 Google Scholar
 PUBMED
 EJMED Journal


 Rudi E. L. Procopio
 Google Scholar
 PUBMED
 EJMED Journal