New Sensitive Technique For Estimation Of Estrogen Receptors


  •   Mohamed Ibraheim Aref

  •   Nagah Mohamed Abomohamed

  •   Adel Shalaby


Background: Estrogens play a key role in the human male reproductive system. Estrogen receptors (ERs: ERα and ERβ) was detected in germ cells from spermatogonia to spermatozoa.

Objective: Tto evaluate the different estrogen receptors expressions on the sperm in infertile males using a new technique and apply that on infertile patient with varicocele.

Materials and Methods: This study included 200 males, divided into two equal groups. Group1  included  fertile males, and group II included infertile males tested according to presence of varicocele with different grades which were classified according to Dubin-Amelar clinical classification of varicocele (1970), 1 m pellet was examined for the expression of the ERα and ERβ using immunocytochemistry stain. The stained sperm was processed in Photoshop program. The estrogen receptors were visualized after magnification and increased resolution in addition using a yellow filter. Percentage of stained sperm with different location in relation to the unstained sperm was counted.

Results: There was a strong expression of ER β on the sperm in fertile males while ERα expression very reduced in fertile males and almost absent in all infertile males. Also, there was a statistically significant decrease in both ERα and β expression in infertile males than fertile males and in varicocele.

Conclusion: ER technical estimation using immunoassay was better than any other techniques assay because ER could be present, but not function due to dislocation and deposition into wrong position.

Keywords: Estrogen receptors, infertility, receptor expression, sperm production


Lubahn D.B., Moyer J.S., Golding T.S., Couse J.F., Korach K.S., Smithies O. Alteration of reproductive function but not prenatal sexual development after insertional disruption of the mouse estrogen receptor gene. Proc. Natl. Acad. Sci. USA. 1993;90:11162–11166. doi:10.1073/pnas.90.23.11162.[PMC free article] [PubMed] [CrossRef] [Google Scholar].

Cooper T.G., Noonan E., von Eckardstein S., Auger J., Baker H.W., Behre H.M., Haugen T.B., Kruger T., Wang C., Mbizvo M.T., et al. World health organization reference values for human semen characteristics. Hum. Reprod. Update. 2010;16:231–245. doi: 10.1093/humupd/dmp048. [PubMed] [CrossRef] [Google Scholar].

Schagdarsurengin U., Western P., Steger K., Meinhardt A. 2016 Developmental origins of male subfertility: Role of infection, inflammation, and environmental factors. Semin.Immunopathol.;38:765–781. doi: 10.1007/s00281-016-0576-y. [PubMed] [CrossRef] [Google Scholar].

Bois C., Delalande C., Nurmio M., Parvinen M., Zanatta L., Toppari J., Carreau S. Age- and cell-related gene expression of aromatase and estrogen receptors in the rat testis. J. Mol. Endocrinol. 2010;45:147–159. [PubMed] [Cross Ref] [Google Scholar].

Lucas T.F., Lazari M.F., Porto C.S. Differential role of the estrogen receptors ESR1 and ESR2 on the regulation of proteins involved with proliferation and differentiation of sertoli cells from 15-day-old rats. Mol. Cell. Endocrinol. 2014;382:84–96. doi: 10.1016/j.mce.2013.09.015. [PubMed] [CrossRef] [Google Scholar].

Cheng C.Y., Boettcher B., Rose R.J., Kay D.J., Tinneberg H.R. The binding of sex steroids to human spermatozoa.An autoradiographic study. Int. J. Androl. 1981;4:1–17. doi: 10.1111/j.1365-2605.1981.tb00685.x. [PubMed] [CrossRef] [Google Scholar].

Cheng C.Y., Boettcher B., Rose R.J. Lack of cytosol and nuclear estrogen receptors in human spermatozoa. Biochem.Biophys. Res. Commun. 1981;100:840–846. doi: 10.1016/S0006-291X(81)80250-1. [PubMed] [CrossRef] [Google Scholar].

Vander Borght M1, Wyns C2 Fertility and infertility: Definition and epidemiology. ClinBiochem. 2018 Dec;62:2-10. doi: 10.1016/j.clinbiochem.2018.03.012. Epub 2018 Mar 16.

Sirianni R., Chimento A., Ruggiero C., De Luca A., Lappano R., Ando S., Maggiolini M., Pezzi V. The novel estrogen receptor, G protein-coupled receptor 30, mediates the proliferative effects induced by 17β-estradiol on mouse spermatogonial GC-1 cell line. Endocrinology. 2008;149:5043–5051. doi: 10.1210/en.2007-1593. [PubMed] [CrossRef] [Google Scholar].

Sheehan M Ramasamy R &Lamb D. Molecular mechanisms involved in varicocele-associated infertility. Journal of Assisted Reproduction and Genetics March 2014. 31(5) DOI: 10.1007/s10815-014-0200-9PubMed.

Brigitta D, Bálint C. and Melinda E. Heat-Shock Proteins in Neuro-inflammation. Front Pharmacol. 2019; 10: 920.. doi: 10.3389/fphar.2019.00920 PubMed.

Lanneau D, Brunet M. Frisan E, Solary E, Fontenay M, Garrido C. 2008Heat shock proteins: essential proteins for apoptosis regulation. J Cell Mol Med. 2008 Jun;12(3):743-61. doi: 10.1111/j.1582-4934.2008.00273.x. Epub Feb 8.

Mjahed H, Girodon F, Fontenay M, GarridoHeat shock proteins in hematopoietic malignancies. C.Exp Cell Res. 2012 Sep 10; 318(15):1946-58. Epub 2012 May 28.

Vedeckis W.V., Ali M.. Allen H.R.cancer Res. Regulation of glucorticoid receptor proteinand, RNA levels 1989 Apr 15;49(8 Suppl):2295s-2302s.

Agarwal A, Sharma RK, Nallella KP, et al; Reactive oxygen species as an independent marker of male factor infertility.SikkaSCFertilSteril. 2006 Oct;86(4):878-85.

Desai N, Sharma R, Makker K, Sabanegh E, AgarwalA.FertilSteril Physiologic and pathologic levels of reactive oxygen species in neat semen of infertile men. 2009 Nov; 92(5):1626-31. Epub 2008 Oct 19.

Hu X,Zheng Ding Z, Hong Z, et al; Spermatogenesis improved by suppressing the high level of endogenous gonadotropins in idiopathic non-obstructive azoospermia: a case control pilot study Reprod Bio lEndocrinol. 2018; 16: 91.. doi: 10.1186/s12958-018-0401-7 PMCID: PMC6150963 PMID: 30243299.

Hussein A, Ozgok Y, Ross L, Rao P, Niederberger C. BJU Int. 2013 Mar; 111(3 Pt B):E110-4. Epub 2012 Sep 7.

Shiraishi K, Matsuyama H. Endocr J. 2017 Feb 27; 64(2):123-131. Epub 2017 Jan 19.

Macdonald AA, Stewart AW, Farquhar CM. Hum Reprod. 2013 Dec; 28(12):3178-87. Epub 2013 Oct 15.

Ma J, Wu L, Zhou Y, Zhang H, Xiong C, Peng Z, Bao W, Meng T, Liu Y. Hum Reprod. 2019 Jan 1; 34(1):155-162.

Fejes I, Koloszár S, Szöllosi J, Závaczki Z, Pál A. Is semen quality affected by male body fat distribution?.Andrologia. 2005 Oct;37(5):155-9. PMID:16266392 DOI:10.1111/j.1439-0272.2005.00671.x.

Lu JC, Jing J, Dai JY, Zhao AZ, Yao Q, Fan K, Wang GH, Liang YJ, Chen L, Ge YF, et al.Body mass index, waist-to-hip ratio, waist circumference and waist-to-height ratio cannot predict male semen quality: a report of 1231 subfertile Chinese men.Andrologia. 2015 Nov; 47(9):1047-54. Epub 2014 Nov 22.

Rufus O, James O and Michael A. Male obesity and semen quality: Any association? Int J Reprod Biomed (Yazd). 2018 Apr; 16(4): 285–290. PMCID: PMC6004593PMID: 29942937.

Hart K, Tadros NN The role of environmental factors and lifestyle on male reproductive health, the epigenome, and resulting offspring. Panminerva Med. 2019 Jun;61(2):187-195. doi: 10.23736/S0031-0808.18.03531-0.


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How to Cite
Aref, M., Abomohamed, N., & Shalaby, A. (2020). New Sensitive Technique For Estimation Of Estrogen Receptors. European Journal of Medical and Health Sciences, 2(3).