A Comparative in Vivo Study on Bambusa Polymorpha, Mentha Piperita and Clitoria Ternatea as Alternative Anxiolytic


  •   Tasnuva Tunna

  •   Mst. Sharmin Akter

  •   Mahmuda Parvin

  •   Md. Jilhaz

  •   Shamema Jahan

  •   Zaidul ISM


This is a continuation of our “Stress Management with Herbal Alternatives” project whereby we are testing traditionally used herbs to scientifically establish their efficacy as anxiolytics for the 1st time. For the current study Bamboo leaf tea, Mint leaf tea and Clitoral bush petal tea were compared for their anxiolytic potency. Male Swiss albino mice were used to test on Open Field test (OFT) and Elevated Plus Maze (EPM) anxiety model. Diazepam (at the dose of 0.25mg/kg body weight) was used as  standard and Bangladeshi indigenous bamboo aka Bambusa polymorpha or Bamboo, Mentha piperita or Mint and Clitoria ternatea or Clitoral Bush (at doses of 1g/kg body weight) were the sample. The control group had no intervention. For OFT we tested line crossing, time spent in center, time spent in thigmotaxis and for EPM we measured the number of entry in open and closed hands, time spent in the open and closed hands. Parameters such as rearing, grooming, urination and defecation were also monitored for both tests.The trial results showed Clitoral bush and Mint leaves to have greater anxiolytic activity than Bamboo and Diazepam. Bamboo and Clitoral Bush helped in weight loss, wherelse Diazepam caused weight gain. Based on all criteria, Clitoral bush tea showed highest potential to reduce stress as well as stress related obesity. Results were followed by mint, diazepam and bamboo respectively in their potency for anxiolytic effect.

Keywords: Stress management, Bamboo leaf tea, Clitoria Ternatea, Holistic Anxiolytic, Mint tea


Glavin, G.B. Stress and brain noradrenaline: a review. Neuroscience & Biobehavioral Reviews, 1985, 9(2), 233-243.

Khansari, D.N.; Murgo, A.J.; Faith, R. E. Effects of stress on the immune system. Immunology Today, 1990, 11, 170-175.

Steptoe, A.; Hamer, M.; Chida, Y. The effects of acute psychological stress on circulating inflammatory factors in humans: a review and meta-analysis. Brain, Behavior, and Immunity, 2007, 21(7), 901-912.

Scott, S.B.; Graham-Engeland, J.E.; Engeland, C.G.; Smyth, J.M.; Almeida, D.M. The effects of stress on cognitive aging, physiology and emotion (ESCAPE) project. BMC Psychiatry, 2015, 15, 146.

Innes, K.E.; Vincent, H.K. The influence of yoga-based programs on risk profiles in adults with type 2 diabetes mellitus: a systematic review. Evidence-Based Complementary and Alternative Medicine, 2007, 4(4), 469-486.

Brown, R.P.; Gerbarg, P.L. Sudarshan Kriya yogic breathing in the treatment of stress, anxiety, and depression: part I—neurophysiologic model. Journal of Alternative & Complementary Medicine, 2005, 11(1), 189-201.

Tunna,T,S. Complementary and alternative medicinal approach to stress management. Frontiers Drug Chemistry Clinical Research, 2018, DOI: 10.15761/FDCCR.1000113

Zhang, Y.; Yao, X.; Bao, B.; Zhang, Y. Anti‐fatigue activity of a triterpenoid‐rich extract from Chinese bamboo shavings (Caulis bamfusae in taeniam). Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 2006, 20 (10), 872-876.

Rathod, J.D.; Pathak, N.L.; Patel, R.G.; Jivani, N.P.; Bhatt Nayna, M. Phytopharmacological properties of Bambusa arundinacea as a potential medicinal tree: An Overview. Journal of Applied Pharmaceutical Science, 2011, 1(10), 27-31.

Sun, J.; Yue, Y.; Tang, F.; Guo, X. Simultaneous HPTLC analysis of flavonoids in the leaves of three different species of bamboo. JPC-Journal of Planar Chromatography-Modern TLC, 2010, 23 (1), 40-45.

Iscan, G.; KIrimer, N.; Kürkcüoglu Mn.; Baser, H.C.; Demirci, F. Antimicrobial screening of Mentha piperita essential oils. Journal of Agricultural Food Chemistry, 2002, 50, 3943-3946.

Keifer, D.; Ulbricht, C.; Abrams, T.R.; Basch, E.; Giese, N. Peppermint (Mentha piperita) an evidence-based systematic review by the natural standard research collaboration. Journal of Herbal Medicine, 2008, 7, 91-143.

Akram, M.; Uzair, M.; Malik, N.S.; Mahmood, A.; Sarwer, N.; Madni, A., Asif, H.M. Mentha arvensis Linn.: A review article. Journal of Medicinal Plants Research, 2011, 5(18), 4499-4503.

Tunna, T.S.; Parvin, M.; Akter, M.S.; Jilhaz, M.; Mim, F.A.; Jahan, S.; Zaidul, I.S.M. Rose Petal tea as an axiolytic for stress management: An In Vivo study based on Elevated plus Maze and Open Field Test. International Journal of Pharmacy and Biological Sciences, 2019, 9(2), 587-597

Finkel, R.; Clark, M.A.; Cubeddu, L.X. (Eds.). Pharmacology. Lippincott Williams & Wilkins. Anxiolytic and Hypnotic Drugs, 2009, 9, 89-98.

Jain, N.N.; Ohal, C.C.; Shroff, S.K.; Bhutada, R.H.; Somani, R.S.; Kasture, V.S.; Kasture, S. B. Clitoria ternatea and the CNS. Pharmacology Biochemistry and Behavior, 2003, 75(3), 529-536.

Parveen, T.; Amin, N.; Saleem, D.; Razi, F.; Haider, S.; Haleem, D.J. Antistress effect of Mentha piperita in rats and the role of brain serotonin and dopamine. Asian Journal of Pharmaceutical & Biological Research, 2012, 2(1), 73-78

Hewagalamulage, S.D.; Lee, T.K.; Clarke, I.J.; Henry, B.A. Stress, cortisol, and obesity: a role for cortisol responsiveness in identifying individuals prone to obesity. Domestic Animal Endocrinology, 2016, 56, S112-S120.

Akinmoladun, O.F.; Sabi, R.S.; Adedayo, O.T. Toxicological evaluation of graded levels of freshly harvested bamboo (Bambusa arundinacea) and tridax (Tridax procumbens) leaves on blood chemistry of rabbits. Nigerian Journal of Animal Production, 2018, 45(1), 183 – 197.

Thamizharasan.S.; Umamaheswari.S.; Rajeswari, Hari.; Ulagaratchagan.V. Quantitative Phytochemical Analysis of Bambusa arundinacea Seeds. The International Journal of Pharmacognosy and Phytochemical Research, 2015, 7(5), 980-983.

Trevisan, S.C.C.; Menezes, A.P.P.; Barbalho, S. M.; Guiguer, É.L. Properties of Mentha piperita: a brief review. World Journal of Pharmacy and Medical Research, 2017, 3(1), 309-13.

Keifer D,; Ulbricht C,; Abrams T.R,; Basch, E.; Giese, N. Peppermint (Mentha piperita) an evidence-based systematic review by the natural standard research collaboration. Journal of Herbal Medicine, 2008, 7, 91-143.

Saeidnia S,; Gohari AR,; Yassa N,; Shafiee A. Composition of the volatile oil of achillea conferta dc. From Iran. DARU, 2005, 13, 34-36.

Kazuma, K.; Noda, N.; Suzuki, M. Malonylated flavonol glycosides from the petals of Clitoria ternatea. Phytochemistry, 2003, 62(2), 229-237.

Kumar, V.; Mukherjee, K.; Kumar, S.; Mal, M.; Mukherjee, P.K. Validation of HPTLC method for the analysis of taraxerol in Clitoria ternatea. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques, 2008, 19(3), 244-250.

Taur, D.J.; Patil, R.Y. Evaluation of antiasthmatic activity of Clitoria ternatea L. roots. Journal of ethnopharmacology, 2011, 136(2), 374-376.


Download data is not yet available.


How to Cite
Tunna, T., Akter, M. S., Parvin, M., Jilhaz, M., Jahan, S., & ISM, Z. (2020). A Comparative in Vivo Study on Bambusa Polymorpha, Mentha Piperita and Clitoria Ternatea as Alternative Anxiolytic. European Journal of Medical and Health Sciences, 2(3). https://doi.org/10.24018/ejmed.2020.2.3.266