Use of Alternative Indicator Organisms for The Microbiological Assay of Four Antibiotics Commonly Sold in Uyo, Nigeria

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  •   Chibuzor Nneamaka Nwosu

  •   Chinweizu Ejikeme Udobi

  •   Darlington Ugochukwu Onele

  •   Gideon Ememobong Asuquo

Abstract

Background: The accurate measurement of antibiotics potency is essential for quality checks, and microbiological assays have been used extensively for this purpose. Unfortunately, in most places especially in the developing countries, the indicator organisms recommended for these assays are difficult to obtain as there are no culture collection centers that keep them. Alternative indicator organisms which will meet the specifications of the reference books were therefore sought for and used in the microbiological assay of some commonly used antibiotics. Pure powders of four commonly used antibiotics to be assayed were obtained and confirmed using the Fourier infrared spectroscopy. Nine strains of organisms which are Escherichia coli WG5, Enterococcus duranss ATCC 11576 and Staphylococcus aureus NCTC6571, Pseudomonas aersuginosa ATCC 27853, Enterococcus gallinarium ATCC 49573, Enterococcus faecium ATCC 49185, Stenotrophomonas maltophilia ATCC 13637, Aeromonas hydrophilia ATCC 7966 and Listeria monocytogenes were randomly selected and screened for their suitability to be used based on their susceptibility to the antibiotics to be assayed, growth characteristics, linearity on a dose response curve and pathogenicity. Selected organisms were eventually used as indicator organisms in the microbiological assay of different brands of Ampicillin, Amoxicillin, Tetracycline and Erythromycin obtained in Uyo, Nigeria using the 6x6(3+3) Latin square design on assay plates.


Results: Results obtained confirmed the standard antibiotic powders and showed that Staphylococcus aureus NCTC6571 could be used to assay Amoxicillin while Enterococcus durans ATCC 11576 and Escherichia coli WG5 could be used to assay Erythromycin, Ampicillin. and Tetracycline Results further showed that the antibiotics assayed had percentage potency of between 104 and168%. All assayed products were within acceptable limits confirming all the antibiotics assayed to be of good quality, except the amoxicillin capsule which exceeded the percentage potency limit allowed.


Conclusion: It is concluded that alternative indicator organisms that meet the specifications of reference books can be used for the microbiological assay of antibiotics in the absence of those recommended.


Keywords: Antibiotics, assay, indicator organisms, microbiological, recommended

References

Udobi CE, Onaolapo JA. Microbiological assay of active components of ampicillin in ampicillin /chocacillin suspension using BacullusMegathanum NCTC 10342A76 as indicator organism. Afr. J. Mic. Res. 2010; 4(1): 51-54.

Lourenco FR, PintoTJA. Comparison of three environmental design employed in gentamycin on Microbiological assay through agar diffusion. J. Pharm. Sci. 2009; 45: 559-566.

Dafale NA, Semwal UP, Rajput RK, Singh GN. Selection of appropriate analytical tools to determine the potency and bioactivity of antibiotics and antibiotic resistance. J. Pharm. Anal. 2016: 207-2013.

Cazegey ECL, Salgado HRN. Development validation of a Microbiology agar assay for determination germination of Orbifloxacin in Pharmaceutical Preparation, Pharmaceutics. National institute of health, 2011; 3(3): 572-581.

Dafale NA, Senwal UP, Agarwal PK. Quantification of ceftriocone Sodium in Pharmaceutical Preparation by now validated microbiological bioassay Analytical Methods. J. Pharm. Anal. 2012; 4(1): 2490-2498.

Baired RN, Hodges NA, Denyer SP. Handbook of Microbiological Quality Control: Pharmaceuticals and Medical Devices, CRCPress, Boca Raton, FL, USA. 2000.

Nicolle FR, Carlos ER, De P, Daniel MB, Miguel de la G, Salvador GC, et al. Novel approach for the determination of azithromycin in pharmaceutical formulations by Fourier transform infrared spectroscopy in film-through transmission mode. Microchem. Journ. 2013; 110(2013): 301-307.

Clinical and Laboratory Standards Institute (CLSI) Performance standards for antimicrobial susceptibility testing. 26th edition, CLSI supplement M100s. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania. 2016.

Udobi, CE. Antibacterial activities and phytochemical analysis of the Leaf, Stem bark and root of the African Locust Bean (Parkia biglobosa) (Jacq) Benth (Mimosaceae). PhD. Thesis. Ahmadu Bello.University; 2008.

Awe S, Amobi OO. Antibacterial, Phytochemical and Proximate Analysis of Pteridium aquilinum. Inter. J. Res. Pharm. Biosci. 2015; 2(9): 1-7.

Maria LM, Danielle AA, Jaison CM, Cleber AS. A fully validated Microbiological assay to evaluate the Potency of Ceftriaxone Sodium. Braz. J. Pharm, Sci. 2013; 49(4): 1-10.

Udobi CE, Ubulom PME, Akpabio EI. Comparative studies of the spectrophotometric and microbiological assay method used for the determination of Ampicillin levels in Ampicillin/cloxacillin combination. Curr. Res. J. Biol. Sci. 2011; 3(3): 31-34.

Shah VP, Midha KK, Findlay JWA, Hill HM, Hulse JD, McGilveray IJ, et al. Bioanalytical method validation — a revisit with a decade of progress. Pharm. Res. 2000; 12: 1551–1557.

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How to Cite
Nwosu, C. N., Udobi, C. E., Onele, D. U., & Asuquo, G. E. (2022). Use of Alternative Indicator Organisms for The Microbiological Assay of Four Antibiotics Commonly Sold in Uyo, Nigeria. European Journal of Medical and Health Sciences, 4(3), 112–118. https://doi.org/10.24018/ejmed.2022.4.3.1358