Haematological and Biochemical Safety Evaluation of the Aqueous Fraction of the Methanol Extract of the Leaf of Musa paradisiaca Linn


  •   Ememobong Gideon Asuquo

  •   Chinweizu Ejikeme Udobi


The knowledge of the safety of a medicinal plant is important before it can be administered as a pharmacological agent in either animal or man. Thus, the effect of the aqueous fraction of the methanol extract of the leaf of Musa paradisiacal L. on the haematological and biochemical indices of experimental Swiss albino mice was studied. The dose of the extract that killed 50% of the animal population (LD50) was determined using the Miller and Tinter method while the effect of the extract on the biochemical and haematological indices of wistar rats was determined after 21 days of administration of three different doses of the extract namely low, medium and high doses which are 10, 20 and 30% of the obtained LD50. Results obtained showed an LD50 of 5754.40±694.25 mg/kg of body weight indicating that the leaf of Musa paradisiaca is nontoxic. Results further showed that the extract had no significant (p<0.05) effect on the total protein, albumin, globulin, urea, AST and ALP levels while creatinine and ALT levels were slightly decreased. Likewise, haematological parameters such as RBC, HB, PCV, RBC differentials and platelet counts were not significantly (p<0.05) affected while WBC level was slightly reduced. The body weights of the experimental animals taken on the first day of the test, ten days after and on the last day of the test showed that the changes in the body weight of the animals were directly proportional to the number of treatment days. It is concluded that the leaf of Musa paradisiaca has no toxic effect on the haematological and biochemical parameters of the test animals hence it can be safe for use as a pharmacological agent.

Keywords: Biochemical, haematological, medicinal plant, Musa paradisiaca, pharmacological


Ravanbakhsh AMM, Jalilzade-Amin G, Javadi S, Maham M, Mohammadnejad D, Rashidi MR. Acute and subchronic toxicity study of the median septum of Juglans regia in Wistar Rats. Advanced Pharmaceutical Bulletin. 2016; 6(4): 541-549.

Chinedu E. Arome D, Ameh FS, Jacob DL. An Approach to Acute, Subacute, Subchronic and Chronic Toxicity Assessment in Animal Models. Toxicology International. 2015; 22(2): 83-87.

Arome D, Chinedu E. The importance of toxicity testing. Journal of Pharmaceutical and Biosciences. 2013; 4: 146-148.

Ghani, A. Medicinal Plants of Bangladesh: Chemical Constituents and Uses. 2nd Edition. The Asiatic Society of Bangladesh, Dhaka, Bangladesh. 2003: 315.

Imam MZ, Akter S. Musa paradisiaca L. and Musa sapientum L.: A Phytochemical and Pharmacological Review. Journal of Applied Pharmaceutical Science. 2011; 1(5): 14-20.

Yusuf M, Begum J, Hoque MN, Chowdhury JU. Medicinal plants of Bangladesh, Bangladesh Council Science and Industrial Resources. 2009.

Krishnan K, Vijayalakshmi NR. Alterations in lipids & lipid peroxidation in rats fed with flavonoids rich fraction of banana (Musa paradisiaca) from high background radiation area. Indian Journal of Medical Resources.2005; 122: 540-546.

Bera S, Bhattacharya S, Pandey JN, Biswas M. Acute and sub-chronic toxicity study of Musa paradisiaca leaf extracts in mice. Journal of Advanced Pharmacy Education & Research. 2013; 3(2): 90-93.

Ugbogu EA, Ude VC, Elekwa I, OkuuArunsi, U, Uche-Ikonne C, Nwakanma C. Toxicological profile of the aqueous-fermented extract of Musa paradisiaca in rats. African Journal of Phytomedicine. 2018; 8(6): 478-487.

Udobi CE, Onaolapo JA, Agunu A. Antibacterial Activities and Bioactive components of aqueous fraction of the stem bark of Parkia biglobosa(Jacq) (Mimosacea). Nigerian Journal of Pharmaceutical Science. 2008; 7: 76-82.

Randhawa, MA. Calculation of LD50 Values from the Method of Miller and Tainter, 1944. Journal of Ayub Medical College Abbottabad. 2009; 21(3): 184-185.

Statistical Package for the Social Sciences (SPSS) version 23.

Teke GN, Kuete V. Acute and subacute toxicities of African medicinal plants. Toxicological Survey of African Medicinal Plants. 2014; 63-98.

Arsad SS, Esa NM, Hamzah H, Othman F. Evaluation of acute, sub-acute and sub-chronic oral toxicity of Rhaphidophora decursiva (Roxb.) Schott extract in male Sprague Dawley rats. Journal of Medicinal Plant Research. 2013; 7(4): 3031-3040.

Udobi CE, Umoh BE, Asuquo EG. Biochemical and Haematological safety evaluation of the ethanol extract of the stem bark of Blighia sapida using Swiss albino mice. Journal of Bioscience and Biotechnology Discovery. 2017; 2: 21-28.

Olorunnisola O, Bradley G, Afolayan A. Acute and sub-chronic toxicity studies of methanolic extract of Tulbaghia violacea rhizomes in Wistar rats. African Journal of Biotechnology. 2012; 11: 14934-14940.

Sreedharan R, Devarajan P, Van Why S. Pathogenesis of acute renal failure. In: Avner ED, Harmon WE, Niaudet P, Yoshikawa N, editors. Pediatric Nephrology. Heidelberg, Germany: Springer-Verlag. 2009: 1579-602.

Chavda R, Vadalia K, Gokani RL Hepatoprotective and antioxidant activity of root bark of Calotropis procera R. Br (Asclepediaceae). International Journal of Pharmacology. 2010; 6: 937-943.

Al-Mamary M, Al-Habori M, Al-Aghbari, AM, Baker MM. Investigation into the toxicological effects of Catha edulis leaves: a short -term study in animals. Phytotherapy Resources. 2002; 16: 127–132.

Adedapo AA, Abatan MO, Olorunsogo OO. Toxic effects of some plants in the Genus Euphorbia haematological and biochemical parameters of rats. Veterinary Archives. 2004; 74(2): 53-62.

Harizal SN, Mansor SM, Hasnan J, Tharakan JKJ, Abdullah J. Acute toxicity study of the standardized methanolic extract of Mitragyna speciosa Korth in Rodent. Journal of Ethnopharmacology. 2010; 131: 404-409.

Michael B, Yano B, Sellers RS, Perry R, Morton D, Roome N, et al. Evaluation of organ weights for rodent and non-rodent toxicity studies: a review of regulatory guidelines and a survey of current practices. Toxicology and Pathology. 2007; 35: 742-750.


Download data is not yet available.


How to Cite
Asuquo, E. G., & Udobi, C. E. (2022). Haematological and Biochemical Safety Evaluation of the Aqueous Fraction of the Methanol Extract of the Leaf of Musa paradisiaca Linn. European Journal of Medical and Health Sciences, 4(3), 46–51. https://doi.org/10.24018/ejmed.2022.4.3.1322

Most read articles by the same author(s)